B-h4-1BB/hHER2 mice

C57BL/6-Tnfrsf9tm1(TNFRSF9)Bcgen Erbb2tm1(ERBB2)Bcgen/Bcgen • 111869

B-h4-1BB/hHER2 mice

Product nameB-h4-1BB/hHER2 mice
Catalog number111869
Strain nameC57BL/6-Tnfrsf9tm1(TNFRSF9)Bcgen Erbb2tm1(ERBB2)Bcgen/Bcgen
Strain backgroundC57BL/6
NCBI gene ID (Human)
AliasesILA; 4-1BB; CD137; CDw137; IMD109; NEU; NGL; HER2; TKR1; CD340; HER-2; VSCN2; MLN 19; MLN-19; c-ERB2; c-ERB-2; HER-2/neu; p185(erbB2)

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  • Phenotypic analysis
  • Efficacy

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    发表文章

      mRNA expression analysis

      Strain specific analysis of 4-1BB (TNFRSF9) and HER2 (ERBB2) gene expression in wild-type mice and B-h4-1BB/hHER2 mice by RT-PCR. Mouse Tnfrsf9 mRNA was detectable only in spleen of wild-type mice (+/+). Human TNFRSF9 mRNA was detectable only in spleen of homozygous B-h4-1BB/hHER2 mice (H/H;H/H), but not in wild-type mice. Mouse Erbb2 mRNA was detectable only in liver of wild-type mice (+/+). Human ERBB2 mRNA was detectable only in liver of homozygous B-h4-1BB/hHER2 mice (H/H;H/H), but not in wild-type mice.

      Protein expression analysis
      Strain specific 4-1BB expression analysis in homozygous B-h4-1BB/hHER2 mice by flow cytometry.Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-h4-1BB/hHER2 mice (H/H;H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-4-1BB antibody. Mouse 4-1BB was detectable in WT mice (+/+). Human 4-1BB was exclusively detectable in homozygous B-h4-1BB/hHER2 mice (H/H;H/H) but not in WT mice (+/+).
      IHC analysis of HER2 expression
      Immunohistochemical (IHC) analysis of HER2 expression in homozygous B-h4-1BB/hHER2 mice.The liver and mammary gland were collected from WT mice and homozygous B-h4-1BB/hHER2 mice and analyzed by IHC with anti-HER2 antibody. HER2 was detectable in WT mice and homozygous B-h4-1BB/hHER2 mice due to the cross-reactivity of the antibody. The arrow indicates tissue cells with positive HER2 staining (brown).
      In vivo efficacy of anti-human HER2 ADC Trastuzumab analog-MMAE (in-house)

      Antitumor activity of anti-human HER2 ADC (Trastuzumab analog-MMAE, in-house) in B-h4-1BB/hHER2 mice. (A) Anti-human HER2 ADC inhibited B-hHER2 MC38 plus tumor growth in B-h4-1BB/hHER2 mice. Murine colon cancer B-hHER2 MC38 plus cells were subcutaneously implanted into homozygous B-h4-1BB/hHER2 mice (female, 8-week-old, n=6). Mice were grouped when tumor volume reached approximately 100-150 mm3, at which time they were intravenously injected with anti-human HER2 ADC Trastuzumab analog-MMAE (in-house) indicated in panel. (B) Body weight changes during treatment. As shown in panel A, 10mg/kg anti-human HER2 ADC was efficacious in controlling tumor growth in B-h4-1BB/hHER2 mice, demonstrating that B-hHER2 MC38 plus provide a powerful preclinical model for in vivo evalsuation of anti-human HER2 ADC. Values are expressed as mean ± SEM.

      * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-h4-1BB/hHER2 mice] (Cat# 111869) was purchased from Biocytogen.
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